Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography

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For the first time, the three-dimensional (3D) internal structure of naturally produced Didymosphenia geminata frustules were nondestructively visualized at sub-100 nm resolution. The well-optimized hierarchical structures of these natural organisms provide insight that is needed to design novel, environmentally friendly functional materials. Diatoms, which are widely distributed in freshwater, seawater and wet soils, are well known for their intricate, siliceous cell walls called ‘frustules’. Each type of diatom has a specific morphology with various pores, ribs, minute spines, marginal ridges and elevations. In this paper, the visualization is performed using nondestructive nano X-ray computed tomography (nano-XCT). Arbitrary cross-sections through the frustules, which can be extracted from the nano-XCT 3D data set for each direction, are validated via the destructive focused ion beam (FIB) cross-sectioning of regions of interest (ROIs) and subsequent observation by scanning electron microscopy (SEM). These 3D data are essential for understanding the functionality and potential applications of diatom cells.

Tytuł
Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography
Twórca
Zgłobicka Izabela
Słowa kluczowe
Didymosphenia geminata; diatoms; okrzemki
Współtwórca
Li Qiong
Gluch Jürgen
Płocińska Magdalena
Noga Teresa
Dobosz Romuald
Szoszkiewicz Robert
Witkowski Andrzej ORCID 0000-0003-1714-218X
Zschech Ehrenfried
Kurzydłowski Krzysztof J.
Data
2017
Typ zasobu
artykuł
Identyfikator zasobu
DOI 10.1038/s41598-017-08960-5
Źródło
Scientific Reports, 2017, vol. 7, 9086
Język
angielski
Prawa autorskie
CC BY CC BY
Kategorie
Publikacje pracowników US
Data udostępnienia13 maj 2022, 14:13:53
Data mod.13 maj 2022, 14:13:53
DostępPubliczny
Aktywnych wyświetleń0