Study on biogenic spindle-shaped iron-oxide nanoparticles by Pseudostaurosira trainorii in field of laser desorption/ionization applications

CC BY Logo DOI

Nanostructures-assisted laser desorption/ionization mass spectrometry (NALDI-MS) is gaining attention for the analysis of a wide range of molecules. In this present investigation, Pseudostaurosira trainorii mediated biosynthesized iron-oxide nanoparticles (IONPs) have been utilized as nanostructures assisting ionization and desorption for laser desorption/ionization mass spectrometry (LDI-MS). The chain forming diatom, P. trainorii showed efficiency in the production of IONPs against 0.01 M Fe+3 (pH 2) aqueous solution at the intracellular and extracellular level. The whole biomass and external media turned dark orange in color after 3 days of reaction with Fe3+ solution. Scanning electron microscopic (SEM) images illustrated that the surface of Fe3+ exposed frustules of P. trainorii were entirely covered by synthesized nanostructures contrasting with the natural surface ornamentation of control cells. The IONPs loaded frustules also exhibited catalytic properties by decolorizing yellow colored nitrophenol after 3 h of reaction. Transmission electron microscopic (TEM) images confirmed that the produced particles are spindle-shaped with ~50–70 nm length and ~10–30 nm width. The biogenic IONPs were utilized as an inorganic matrix in LDI-MS and showed high sensitivity towards small molecules as glucose, alanine and triacylglycerols at nano- and picomolar level per spot, respectively. The presented biocompatible technique offers new perspectives in nanobiotechnology for the production of spindle-shaped IONPs that can be applied in future for the preparation of NALDI plates.

Tytuł
Study on biogenic spindle-shaped iron-oxide nanoparticles by Pseudostaurosira trainorii in field of laser desorption/ionization applications
Twórca
Roychoudhury Piya ORCID 0000-0002-6368-5428
Słowa kluczowe
iron-oxide nanoparticles; biogenesis; diatom; LDI-MS; nano-spindle
Słowa kluczowe
nanocząstki tlenku żelaza; biogeneza; okrzemka; cząstki o kształcie wrzecionowatym
Współtwórca
Golubeva Aleksandra ORCID 0000-0003-0214-1707
Dąbek Przemysław ORCID 0000-0002-3736-3011
Pryshchepa Oleksandra
Sagandykova Gulyaim
Pomastowski Paweł
Gloc Michał
Dobrucka Renata
Kurzydłowski Krzysztof
Buszewski Bogusław
Witkowski Andrzej ORCID 0000-0003-1714-218X
Data
2022
Typ zasobu
artykuł
Identyfikator zasobu
DOI 10.3390/ijms231911713
Źródło
International Journal of Molecular Sciences, 2022, vol. 23 iss. 19, [br. s.], 117113
Język
angielski
Prawa autorskie
CC BY CC BY
Dyscyplina naukowa
Dziedzina nauk ścisłych i przyrodniczych; Nauki o Ziemi i środowisku
Kategorie
Publikacje pracowników US
Data udostępnienia28 gru 2022, 13:51:15
Data mod.28 gru 2022, 13:51:15
DostępPubliczny
Aktywnych wyświetleń0