Proteomic analysis of murine bone marrow very small embryonic-like stem cells at steady-state conditions and after in vivo stimulation by nicotinamide and follicle-stimulating factor reflects their germ-lineage origin and multi germ layer differentia

CC BY Logo DOI

Very small embryonic-like stem cells (VSELs) are a dormant population of development early stem cells deposited in adult tissues that as demonstrated contribute to tissue/organ repair and regeneration. We postulated developmental relationship of these cells to migrating primordial germ cells (PGCs) and explained the quiescent state of these cells by the erasure of differently methylated regions (DMRs) at some of the paternally imprinted genes involved in embryogenesis. Recently, we reported that VSELs began to proliferate and expand in vivo in murine bone marrow (BM) after exposure to nicotinamide (NAM) and selected pituitary and gonadal sex hormones. In the current report, we performed proteomic analysis of VSELs purified from murine bone marrow (BM) after repeated injections of NAM + Follicle-Stimulating Hormone (FSH) that in our previous studies turned out to be an effective combination to expand these cells. By employing the Gene Ontology (GO) resources, we have performed a combination of standard GO annotations (GO-CAM) to produce a network between BM steady-state conditions VSELs (SSC-VSELS) and FSH + NAM expanded VSELs (FSH + NAM VSELs). We have identified several GO biological processes regulating development, organogenesis, gene expression, signal transduction, Wnt signaling, insulin signaling, cytoskeleton organization, cell adhesion, inhibiting apoptosis, responses to extra- and intracellular stimuli, protein transport and stabilization, protein phosphorylation and ubiquitination, DNA repair, immune response, and regulation of circadian rhythm. We report that VSELs express a unique panel of proteins that only partially overlapped with the proteome of BM – derived hematopoietic stem cells (HSCs) and hematopoietic mononuclear cells (MNCs) and respond to FSH + NAM stimulation by expressing proteins involved in the development of all three germ layers. Thus, our current data supports further germ-lineage origin and multi germ layer differentiation potential of these cells.

Tytuł
Proteomic analysis of murine bone marrow very small embryonic-like stem cells at steady-state conditions and after in vivo stimulation by nicotinamide and follicle-stimulating factor reflects their germ-lineage origin and multi germ layer differentia
Twórca
Chumak Vira
Słowa kluczowe
nicotinamide; FSH; VSELs; PGCs; proteomics; germ line commitment; UN-SDG3
Współtwórca
Sielatycka Katarzyna ORCID 0000-0003-4272-2266
Ciechanowicz Andrzej ORCID 0000-0002-3804-440X
Bujko Kamila
Ratajczak Mariusz Z.
Kucia Magdalena
Data
2023
Typ zasobu
artykuł
Identyfikator zasobu
DOI 10.1007/s12015-022-10445-6
Źródło
Stem Cells Review and Reports, 2023, vol. 19 iss. 1, pp. 120-132
Język
angielski
Prawa autorskie
CC BY CC BY
Dyscyplina naukowa
Nauki biologiczne; Dziedzina nauk ścisłych i przyrodniczych
Kategorie
Publikacje pracowników US
Data udostępnienia24 lut 2023, 14:24:39
Data mod.24 lut 2023, 14:24:39
DostępPubliczny
Aktywnych wyświetleń0