Prey preference in a kleptoplastic dinoflagellate is linked to photosynthetic performance


Dinoflagellates of the family Kryptoperidiniaceae, known as “dinotoms”, possess diatom-derived endosymbionts and contain individuals at three successive evolutionary stages: a transiently maintained kleptoplastic stage; a stage containing multiple permanently maintained diatom endosymbionts; and a further permanent stage containing a single diatom endosymbiont. Kleptoplastic dinotoms were discovered only recently, in Durinskia capensis; until now it has not been investigated kleptoplastic behavior and the metabolic and genetic integration of host and prey. Here, we show D. capensis is able to use various diatom species as kleptoplastids and exhibits different photosynthetic capacities depending on the diatom species. This is in contrast with the prey diatoms in their free-living stage, as there are no differences in their photosynthetic capacities. Complete photosynthesis including both the light reactions and the Calvin cycle remain active only when D. capensis feeds on its habitual associate, the “essential” diatom Nitzschia captiva. The organelles of another edible diatom, N. inconspicua, are preserved intact after ingestion by D. capensis and expresses the psbC gene of the photosynthetic light reaction, while RuBisCO gene expression is lost. Our results indicate that edible but non-essential, “supplemental” diatoms are used by D. capensis for producing ATP and NADPH, but not for carbon fixation. D. capensis has established a species-specifically designed metabolic system allowing carbon fixation to be performed only by its essential diatoms. The ability of D. capensis to ingest supplemental diatoms as kleptoplastids may be a flexible ecological strategy, to use these diatoms as “emergency supplies” while no essential diatoms are available.

Prey preference in a kleptoplastic dinoflagellate is linked to photosynthetic performance
Yamada Norico
Słowa kluczowe
diatoms; dinotoms; Durinskia capensis; feeding experiments; South Africa
Słowa kluczowe
okrzemki; dinotomy; eksperymenty żywieniowe; Afryka Południowa
Lepetit Bernard
Mann David G.
Sprecher Brittany N.
Buck Jochen M.
Bergmann Paavo
Kroth Peter G.
Bolton John J.
Dąbek Przemysław ORCID 0000-0002-3736-3011
Witkowski Andrzej ORCID 0000-0003-1714-218X
Kim So-Yeon
Trobajo Rosa
Typ zasobu
Identyfikator zasobu
DOI 10.1038/s41396-023-01464-3
The ISME Journal, 2023, t. 17, s. 1578-1588
Prawa autorskie
Dyscyplina naukowa
Nauki o Ziemi i środowisku; Dziedzina nauk ścisłych i przyrodniczych
Publikacje pracowników US
Data udostępnienia29 lis 2023, 08:04:28
Data mod.29 lis 2023, 08:04:28
Aktywnych wyświetleń0